BLITz

Blue Light Inducible TEVprotease

BLITz is a new kind of optogenetic toolkit which enables light-inducible gene-expression in response to specific blue light. BLITz is composed of several protein modules interacting each other.

Serial deletion mutants of BLITz.

In order to screen the best light-inducible BLITz, we generated various serial deletion mutants by replacing the original sequence of the C-terminal of AsLOV2 with the TEVseq. BLITz-2 (AsLOV2 1-136 + TEVseq) has the longest deletion of C-terminal tip of Jαhelix, whereas, BLITz-3 (AsLOV2 1-144 + TEVseq) has the shortest deletion at the C-terminal tip of AsLOV2. In addition, to check the efficiency of TEV protease cleavage sequence (ENLYFQG), single point mutation (Asn→Ala, yellow color) was made from BLITz-1, named BLITz-5 Red colored sequence and molecular structure indicate the conserved TEV protease cleavage sequence. Number in sequence represents the position of amino acids of AsLOV2 domain from its N-terminus.